CRYOKIT V1.0: 3D PRINTED POSITIONAL COOLING PLATFORM
DR. E. HU
CryoKit v1.0 (Fig. 1) is a type of Positional Cooling Platform Devices (PCPD) that can be 3-D printed to produce standard cooling rates with low cost (< $10). Multiple configurations of the raft and height above the liquid nitrogen surface were used to produce a range of cooling rates typically used for fish sperm (i.e., from 4 °C/min to 40 °C/min) (Fig. 2). This was the earliest version of the concept of a 3-D printed PCPD. This device can be assembled within several min with 3-D printed parts (Fig. 3). Several new versions has been developed since the feasibility of CryoKit v1.0 was recognized.